The Proteomics facility offers services for the quantitative analysis of proteins and the characterization of their interactions and post-translational modifications. A Thermo Scientific Q Exactive HF-X Hybrid Quadropole-Orbitrap mass spectometer is available, coupled to a specialized RSLCnano system (Ultimate 3000, Thermo) for peptide separation.

Services

• Differential proteomic analysis of tissues, cell lysates, or other protein sources using label-free quantification

• Differential proteomic analysis using reporter ion labeling (e.g. TMT, iTRAQ)

• Targeted proteomic analysis of specific proteins

• Quantification of panels of human serum proteins

• Identification of protein complex components (e.g. in immunoprecipitates or after affinity chromatography)

• Identification of post-translational modifications on specific proteins

• In-gel digestion and identification of proteins from SDS-PAGE gels

• Meta-proteomic analysis of microbiomes (e.g. in fecal samples; experiments should preferably be complementary to genomic approaches) 

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Sample preparation

Details about sample preparation procedures, amount of sample required, analytical methodology and other details must be discussed in advance with the Unit's contact personnel. 

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