Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap
The Proteomics facility offers services for the quantitative analysis of proteins and the characterization of their interactions and post-translational modifications. A Thermo Scientific Q Exactive HF-X Hybrid Quadropole-Orbitrap mass spectometer is available, coupled to a specialized RSLCnano system (Ultimate 3000, Thermo) for peptide separation.
Differential proteomic analysis of tissues, cell lysates, or other protein sources using label-free quantification
Differential proteomic analysis using reporter ion labeling (e.g. TMT, iTRAQ)
Targeted proteomic analysis of specific proteins
Quantification of panels of human serum proteins
Identification of protein complex components (e.g. in immunoprecipitates or after affinity chromatography)
Identification of post-translational modifications on specific proteins
In-gel digestion and identification of proteins from SDS-PAGE gels
Meta-proteomic analysis of microbiomes (e.g. in fecal samples; experiments should preferably be complementary to genomic approaches)
Details about sample preparation procedures, amount of sample required, analytical methodology and other details must be discussed in advance with the Unit's contact personnel.